Providing a Strategy to Confronting the Salinity Stress by Using the PGPR in a Desert Species (Calligonum comosum L`Her ) in Greenhouse Conditions

Document Type : Original Article

Author

Department of Watershed & Rangeland Management, University of Kashan, Iran

Abstract

The purpose of this study was to evaluating the philological effects of a plant growth regulator in the reduction of salinity stress in the Calligonum comosum L.  In this regard, a factorial randomized complete block design (RCBD) in the greenhouse condition was implemented. The salinity levels included 0 in control, 2.5, 5, 10 ds/m (mild, moderate and severe stress) respectively. Also the PGPR used as a plant growth regulator with concentrations 0 as control, 10-10, 10-8 and 10-6 mmol. After applying the desired treatments for 3 months, total chlorophyll, the yield and antioxidant activity of essential oil in the Calligonum comosum L.  Were measured with international standard methods. The essential oil, extracted by a Clevenger apparatus, and antioxidant activity examined by DPPH method.   The results showed, the highest amount of total chlorophyll was observed was observed in the interaction of control treatment of salinity (0) and 10-8 mmol PGPR with values of 0.85mlg/g. The results of essential oil yield showed although the increase in salinity level reduced the essential oil yield, by using the PGPR could improve the essential oil yield to a satisfactory level. The highest amount of essential oil yield obtained in the mild salinity stress (2.5ds/m) and 10-8 mmol PGPR concentration with values of 0.39g/cm.  The results of antioxidant activity showed by using of PGPR the antioxidant activity improved in all of salinity stress level. Briefly, the results of this study showed that the use of PGPR is a suitable way to cope with the negative effects of salinity stress and increase the physiological performance of Calligonum comosum L. 

Keywords


  1. Abd El-Hadi, N., Abo El-Ala, I.M. and Abd El-Azim, W.M. 2009. Response of some Aristida species to plant growth promoting bacteria isolated from soil rhizosphere. Australian Journal of Basic and Applied Sciences, 3: 4437- 4448.
  2. Abdel-Sattar, EA., Mouneir, SM., Asaad, GF. and Abdallah, HM. 2018. Protective effect of Calligonum comosum on haloperidolinduced oxidative stress in rat. Journal of Toxicol Ind Health. 30:147–153
  3. Ali RM., Abbas, HM. and Kamal, RK. 2007. The effects of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram. Journal of Plant Soil Engineering, 53, 529-43.
  4. AliabadiFarahani, E. and Krima, M. 2004. Stimulation of growth, flowering, biochemical constituents and essential oil of chamomile plant (chamomilla recutita L. With spermidine and stigmasterol application. Journal of Plant Physiology, 30, (1-2): 89-102.
  5. Amakura, Y., Umino, Y., Tsuji, S., Ito, H., Hatano, T., Yoshida, T. and Tonogai, Y. 2002. Constituents and their antioxidative effects in eucalyptus leaf extract used as a natural food additive. Journal of Food Chemistry, 77: 47-56. https://doi.10.1016/S0308-8146(01)00321-1
  6. Arnao, M.B., Cano, A. and Acosta, M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Journal of Food Chemistry Journal of  Food Chemistry.,73, 239–244.
  7. Badria, FA., Ameen, M. and Akl, MR. 2017. Evaluation of cytotoxic compounds from calligonum comosum growing in Egypt. Journal of Naturforsch, 62(9–10):656–660. 
  8. Bahmani, A. 2009. The anatomy of the vegetative organs of three species of the Calligonum genus and chemical composition of essential oils of flowers. Master's thesis Biology (Plant Sciences Trends). Payame Noor University - Tehran Unit. pp: 115. [Persian].
  9. Ben Taarit, MK., Msaada, K., Hosni, M., Hammami, M., Kchouk, E. and Marzouk, B. 2009. Plant growth, essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Journal of  Indian Crops Production, 30, 333-337.
  10. Clouse, S.D., Zurek, D.M., McMorris, T.c. and Baker, M.E. 1992. Effect of brassinolide on gene expression in elongating soybean epicotyls. J Plant Physiology, 100, 1377-1383.
  11. Daneshmandi, M.H. and Azizi, M. 2009. Effects of water stress and mineral zeolite application on quantitative and qualitative characteristics Hungarian cultivars Basil, 6th Iranian Congress of Horticultural Sciences. 25-27 Juley.123-129,
  12. Demir Kaya, M., Gamze, Okc, U., Atak, M. Yakup, C. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europian Journal of of Agriculture, 24, 291–295.
  13. Dow, A.I., Cline, T.A. and Horning, E.V. 1981. Salttolerance studies on irrigated mint. Bulletin of Agricultural Research Center, (Washington State University, Pullman), Washington, 906p.
  14. Faker Baher, Z., Rezai, M.B., Mirza, M. and Abbaszadeh, B. 2011. Quantitative and qualitative evaluation Saturejasahendica, during the drough. Journal of Medicinal and Aromatic Plants Research, 11, 12-22.
  15. Fallahi, J., Koocheki, A. and RezvaniMoghaddam, P. 2009. Effects of biofertilizers on quantitative and qualitative yield of chamomile (Matricaria recutita ) as a medicinal plant. Journal of Agricu Research., 7, 127-135.
  16. Gharib, FA., Moussa, LA. and Massoud, ON. 2008. Effect of compost and biofertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) plant. International Journal of Agriculture Biology. 10, 4: 381-387.
  17. Ingram, J. and Bartels, D. 1996. The molecular basis of dehydration tolerance in plants. J ournal of Plant Physiology., 47, 377-403.
  18. Jafarinia, S., Khosroshahi, V. and Ghasemi, M. 2016. A comprehensive guide and photographer properties and use of medicinal plants. (Sokhangostar press, Tehran),6, 179 p.
  19. Jahan, M., Ghalenoi, SH., Khamoush., A. and Amiri, M.B. 2015. Cheking agro ecological characteristics of Basil (Ocimum basilicum) affected by the use of super absorbent, humic acid and water courses, Journal of Horticuatural Science, 29: 240-254.
  20. Koochaki, A., Tabrizi, L. and Ghorbani, R. 2008. The evaluate the effect of biofertilizers on growth characteristics, yield, quality characteristics medicinal herb hyssop (Hyssopus officinalis). Iranian Agriculture Research Journal. 1:137- 127.
  21. Lucy, M., Reed, E. and Glick, BR. 2004. Applications of free living plant growth-promoting rhizobacteria. Journal of Antoine., 86,1-25.
  22. Mahfouz, S.A. and Sharaf-Eldin, M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare ). Journal of Agricuture sciences. 21, 361- 366.
  23. Mohsen, MA. and Ismail, H. 2016. Response of Calendula officinalis L. which grown in saline soil to Plant Growth Promoters and Some Organic Substances. International Journal of Pharma Technology Research. 9, (4): 153-172.
  24. Mozaffarian, V. 1996. Iran's culture names of plants. (Farhange Moaser press, Tehran), 740 p.
  25. Omidbeygi, R.A. 2009. Aproaches to the production and processing of medicinal plants. (Fekrerooz press, Tehran), 2009, 283p.
  26. Rahmani, N., Aliabadi Farahani, H. and Valadabadi, S. 2008. Effects of nitrogen on oil yield and its component of Calendula (Calendula officinalis ) in drought stress conditions. The world congress on medicinal and aromatic plants, (South Africa), 14-18 June
  27. Rajabi Agra, S.A., Bahmanyar, M., Khavazi., K. and Ramezanpoor, M.R. 2009. The Role of PGPR on some of the indicators of growing rice in saline conditions. First National Con on Env Strin Agr Sci. (Iran, Birjand), 8 - 9 February.
  28. Sabry L., Sattar, MA., Amin, HA. and Sattar, EA. 2016. Antiosteoporotic effect of some herbal extracts versus alendronate on an animal model of osteoporosis. Journal of Life Sciences, 10:177–187.
  29. Swamy, K.N. and Ram, Rao. 2008. SInfluence of 28-homobrassinolid on growth, photosynthesis metabolite and essential oil of geranium .American. Journal of plant physiology, 3., 4173-179.
  30. Tabrizi, L., Koocheki, A. and Ghorbani R. 2008. Effect of biofertilizers on agronomic criteria of Hyssop (Hyssopus officinalis). 2nd Conference of the International Society of Organic Agriculture Research ISOFAR, (Modena, Italy),18-20 June
  31. Tarraf, S. and Ibrim, M.E. 1999. Physiological response of Calligonum comosum plant to brassinosteroid and uniconazole. Culand Pro of Med and Aro Plants. (Academic press, india), 230 p.
  32. Upadhyay, SK., Singh, JS., Saxena, AK. and Singh, DP. 2011. Impact of PGPR inoculation on growth and antioxidant status of wheat under saline conditions. Journal of Plant Biology, 14, 605–611.
  33. Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizer. Journal of Plant and Soil, 255-571-586.
  34. Oliveira, W.P., Souza, C.R., Kurozawa, L. and Park., K.J. 2010. Spray drying of food and herbal products (Wiley press, London), Chapter 5, 113–156
  35. , SC., Cao., ZH., Li, Z.G., Cheung, K.C. and Wong, M.H. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Journal of Geothermal, 125, 155-166.
  36. Zahir, Z.A., Muhammad, A. and Frankenberger, W.T. 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Journal of Advanced Agronomy, 81,97–168.